Science 8 Micro-organisms and a first fermentation

Today groups practiced a complex procedure for investigating the fermentation of a sugar solution by yeast. They first prepared a supply of sugar solution at a specified concentration. They then raised the temperature of the solution to 35 degrees Celcius. 100 ml of the solution was removed and the density determined. The remaining solution was inoculated with a small spoon of dry baker's yeast. 20 ml of the inoculated solution was placed in a fermentation tube. Inoculated solution was added to a plastic pop bottle (about 2/3 to 3/4 of the bottle's capacity.) A deflated balloon was placed over the mouth of the bottle. Three set-ups were completed by the end of the class around 10:00 pm. The following photos were made at 12:00 on the same day. Notice the bubbling and the balloons beginning to inflate.

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